Coding

Part:BBa_K3428000:Design

Designed by: Alexis Choi, Andrew Sillato, Julia Petreczky   Group: iGEM20_Stony_Brook   (2020-10-26)


UV RESISTANCE LOCUS 8 (UVR8 )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 85


Design Notes

This UVR8 came from the native species Arabidopsis thaliana. This protein was modified in that illegal cut sites were removed and replaced with synonymous mutations.

Source

The genome of Arabidopsis thaliana from Uniprot.

References

[1] Camacho, I. S., Theisen, A., Johannissen, L. O., Díaz-Ramos, L. A., Christie, J. M., Jenkins, G. I., Bellina, B., Barran, P., & Jones, A. R. (2019). Native mass spectrometry reveals the conformational diversity of the UVR8 photoreceptor. Proceedings of the National Academy of Sciences, 116(4), 1116–1125. https://doi.org/10.1073/pnas.1813254116

[2] Crefcoeur, R., Yin, R., Ulm, R. et al. Ultraviolet-B-mediated induction of protein–protein interactions in mammalian cells. Nat Commun 4, 1779 (2013). https://doi.org/10.1038/ncomms2800

[3] Müller, K., Engesser, R., Schulz, S., Steinberg, T., Tomakidi, P., Weber, C. C., Ulm, R., Timmer, J., Zurbriggen, M. D., & Weber, W. (2013). Multi-chromatic control of mammalian gene expression and signaling. Nucleic acids research, 41(12), e124. https://doi.org/10.1093/nar/gkt340

[4] Yang, X., Montano, S., & Ren, Z. (2015). How Does Photoreceptor UVR8 Perceive a UV-B Signal?. Photochemistry and photobiology, 91(5), 993–1003. https://doi.org/10.1111/php.12470